Vivaspin 20
5-20 ml samples
The Vivaspin 20ml centrifugal concentrators have been developed to offer increased volume flexibility and performance.
Vivaspin 20 handles up to 20ml in swing bucket centrifuges 14ml in 25° fixed angle rotors accepting 50ml centrifuge tubes. Featuring twin vertical membranes for unparalleled filtration speeds and 100X plus concentrations. Remaining volume is easy to read off the printed scale on the side of the concentrator and the modified dead stop pocket further simplifies direct pipette recovery of the final concentrate.
Process Flexibility
Vivaspin 20 is available with unique accessories and operating methods that are designed to provide more process flexibility and further time saving.
One Step Desalting
In this procedure following concentration, a diafiltration cup is filled with buffer and then spun one time to achieve 98% salt removal. This compares to the need for two spins to achieve the same result with the traditional refill and re-spin procedure.
The improved performance is due to the constant washing action of the buffer solution in the accessory cup as it replaces solvent and salts as they pass through the ultrafiltration membrane.
Gas Pressure Filtration
When an appropriate centrifuge is unavailable, or for single sample processing, Vivaspin 20 can be filled with up to 15ml and then pressurised for bench top concentration. For even faster processing, Gas pressure can be combined with centrifugal force. "Pressure-fugation" is particularly suitable for difficult or viscous samples such as serum, or when using a low process temperature which reduces filtration speed, and generally when minimum process time is essential.
Rotor Compatibility
Please note:
Vivaspin 20 (30 mm x 116 mm) is designed to fit into rotors that can accommodate Falcon 50 ml conical bottom tubes, e.g. Beckman Allegra 25R with TS-5.1-500 swing-out rotor with BUC 5 buckets and 368327 adaptors; Beckman TA-10.250 25° fixed angle rotor with 356966 adaptors; Heraeus Multifuge 3 S-R with (Heraeus / Sorvall) 75006445 swing out rotor with 75006441 buckets and adaptors for Falcon 50 ml conical bottom tubes.
These devices are not designed to fit into rotors that only accept round bottom 29 mm x 105 mm tubes, e.g. Sorvall SS34 or Beckmann JA 20.
If your rotor accepts only 29 mm x 105 mm round bottom tubes, please use the Vivaspin 15, which can be used in either round bottom or conical centrifuge tubes.
Mode | Centrifuge | Centrifuge | Benchtop | Pressure Fuge | ||||
|---|---|---|---|---|---|---|---|---|
Type | Swing Bucket | 25° fixed Angle | Pressure | Swing Bucket | ||||
Pressure/Speed | 3,000 g | 6,000 g | 4 bar | 3,000 g | ||||
Start Volume | 20 ml | 14 ml | 10 ml | 10 ml | ||||
| Min. | Rec. | Min. | Rec. | Min. | Rec. | Min. | Rec. |
Cytochrome c 0.25 mg/ml (12,400 MW) | ||||||||
3,000 MWCO PES | 110 | 97% | 180 | 96% | 60 | 96% | - | - |
BSA 1.0 mg/ml (66,000 MW) | ||||||||
5,000 MWCO PES | 23 | 99% | 29 | 99% | 50 | 98% | 14 | 98% |
10,000 MWCO PES | 16 | 98% | 17 | 98% | 32 | 97% | 8 | 97% |
30,000 MWCO PES | 13 | 98% | 15 | 98% | 32 | 97% | 8 | 97% |
IgG 0.25 mg/ml (160,000 MW) | ||||||||
30,000 MWCO PES | 27 | 97% | 20 | 95% | 46 | 94% | 13 | 97% |
50,000 MWCO PES | 27 | 97% | 22 | 95% | 46 | 93% | 13 | 96% |
100,000 MWCO PES | 25 | 91% | 20 | 90% | 42 | 88% | 12 | 94% |
Latex beads 0.004 % in DMEM +10 % FCS (0.055 µm) | ||||||||
300,000 MWCO PES | 20 | 99% | 35 | 99% | 10 | 99% | - | - |
Latex beads 0.004 % in DMEM +10 % FCS (0.055 µm) | ||||||||
1,000,000 MWCO PES | 4 | 99% | 12 | 99% | 4 | 99% | - | - |
Yeast 1.0 mg/ml (S. Cerevisiae) | ||||||||
0.2 µm Pes | 15 | 95% | 5 | 95% | 20 | 95% | 2 | 95% |
Swing Bucket Rotor | 20 ml |
25° Fixed Angle Rotor | 14 ml |
With Pressure Cap | 15 ml |
Dimensions | |
|---|---|
Total Length | 116 mm |
Width | 30 mm |
Active Membrane Area | 6.0 cm² |
Carrier Required | |
Insert Diameter | 29 mm |
Conical Bottom Tube | 50 ml |
Maximum Centrifugal Force | 8,000 x g |
Maximum Pressure | 5 bar |
Hold up Volume Memb. | <20 µl |
Dead Stop Volume | 50 µl |
Materials of Construction | |
Body and Filtrate Vessel | Polycarbonate |
Concentrator Cap | Polypropylene |
Membranes | Polyethersulfone |
Vivaspin 20 (5 - 20 ml samples) Polyethersulfone Membrane | Pack Size | Prod. No. | Pack Size | Prod. No. |
|---|---|---|---|---|
3,000 MWCO | 12 | VS2091 | 48 | VS2092 |
5,000 MWCO | 12 | VS2011 | 48 | VS2012 |
10,000 MWCO | 12 | VS2001 | 48 | VS2002 |
30,000 MWCO | 12 | VS2021 | 48 | VS2022 |
50,000 MWCO | 12 | VS2031 | 48 | VS2032 |
100,000 MWCO | 12 | VS2041 | 48 | VS2042 |
300,000 MWCO | 12 | VS2051 | 48 | VS2052 |
1,000,000 MWCO | 12 | VS2061 | 48 | VS2062 |
0,2 µm | 12 | VS2071 | 48 | VS2072 |
Start pack | 12 | VS20S1 |
|
|
Centrifuge (Swing Bucket or Fixed Angel) | ||
|---|---|---|
Rotor size (Based on conical bottom centrifuge tubes) | 50 ml (29 mm) | |
Fixed angle rotor (Minimum Angle) | 25° | |
Minimum Effectiv Speed | 1,500 g | |
Preferred Speed | 2,000 - 6,000 x g | |
Optional Pressure Accesories for Vivaspin 20 | Prod. No. | |
Pressure Head with Check Valve | VCA200 | |
Charge Valve for Pressure Head | VCA005 | |
Air Pressure Controller (APC) | VCA002 | |
Concentrate recovery | ||
Fixed or variable volume pipette | Thin gel loader type preferred | |
Vivaspin 20 | Pack Size | Prod. No. |
|---|---|---|
Diafiltration Cups | 12 | VSA005 |
Vivaspin 20 Pressure Head | 1 | VCA200 |
Air pressure Controller (APC) | 1 | VCA002 |
Charge Valve for Pressure | 1 | VCA005 |
Female Coupling | 1 | VCA010 |
Male Tube Coupling | 1 | VCA011 |
4 mm OD Pressure Tube (3m) | 1 | VCA012 |