Vivapure AdenoPACK 20
The optimal kit for construct screening
Vivapure AdenoPACK 20 is the small scale kit in the AdenoPACK series, purifying up to 1 x 1012 viral particles from 20 ml cell culture. Especially when testing new constructs, parallel and fast purifications of different adenoviruses are essential. This kit allows the rapid, simple and affordable spin column based purification of 6 different samples in parallel and bridges a gap in the CsCl density gradient method – for the first time adenovirus can efficiently be purified from less than 100 ml cell culture volume!
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Membrane adsorber technology: |
| Fluorescent cell assay |
Membrane adsorber technology:
AdenoPACK encompasses the advantages of open structures of membranes with the benefits of classical chromatography. Our membrane adsorbers with porous matrices, high capacities, low differential pressures, high flow rates and low unspecific adsorption show an excellent performance in small scale virus purification. However, they are also scalable and confirm to cGMP facilities to large volume, high performance separation, reducing the processing time by a factor of 10 in the final process.
Feature |
| Benefit |
|---|---|---|
Spin column based kit | » | Easy and fast parallel purification of multiple samples |
Virus purification based on membrane adsorber technology | » | High-speed process completed in about 1 hour |
Kit contents include Vivaclear, AdenoPACK Maxi spin columns, Vivaspin and all buffers | » | Convenient, easy to use alternative |
Able to process 6 x 20 ml cell culture | » | Flexible, most cost-effective kit in the market for purifying 6 x 1011-12 viral particles. |
Cultures should be grown in DMEM + 10% FBS pH 7.0 - 7.4 at 37°C with 5% CO2
Kit Specifications | |
|---|---|
Sample Size | Up to 6 x 20 ml of Adenovirus supernatants |
Recovered virus particles (VP) | Typically up to 1 x 1012 per 20 ml preparation |
VP/IU | 20 - 50 |
Processing time | Typically 1 hour |
Endotoxin level | < 0.025 EU/ml |
Materials of construction | |
|---|---|
AdenoPACK spin column housing: | Polypropylene |
AdenoPACK filter membrane | Stabilized regenerated cellulose |
Vivaclear Maxi housing | Polypropylene |
Vivaclear Maxi membrane 0.45 µm | PES |
Vivaspin housing | Polycarbonate |
Vivaspin membrane | PES |
Buffer containers | LDPE |
Kit Contents and ordering information | |
|---|---|
AdenoPACK 20 | VS-AVPQ020 |
AdenoPACK 20 RT | VS-AVPQ022*** |
AdenoPACK Maxi spin columns | 6 |
Benzonase® Nuclease* 12.5 U/µl | 120µl |
Vivaclear Maxi membrane 0.45 µm | 6 |
Empty 50 ml tubes | 6 |
Loading Buffer (10x) | 1 x 25 ml |
Washing Buffer (10x) | 1 x 30 ml |
Elution Buffer | 1 x 20 ml |
Vivaspin 20 concentrator, 100 kDa MWCO | 6 |
Instructions | 1 each for Kit and Vivaspin |
***AdenoPACK 20 RT does not contain Benzonase - all other contents are identical to AdenoPACK 20. | |
Product | Purification method | Process time | Eluate* | Recovery** | Viral Particles |
|---|---|---|---|---|---|
AdenoPACK 20 | 20 ml culture | 1 hour | 1 ml | 65-70% | 1 x 1011-12 |
AdenoPACK 100 | 60 ml culture | 1-2 hours | 1 ml | 65% | 1-3 x 1012 |
AdenoPACK 100 | 200 ml culture | 2 hours | 1 ml | 80% | 1 x 1013 |
AdenoPACK 500 | 500 ml culture | 1-2 hours | 1 ml | 80% | 1-3 x 1013 |
| 500 ml CsCl | 24-48 hours | 1-2 ml | 60-70% | 1-5 x 1011-12 |
*after dialysis **before buffer exchange
Virus purification tests conducted in cooperation with Progen Biotechnik GmbH,Heidelberg.
Providing custom made vector development and production service for Adenovirus and Adeno-associated Virus vector systems.
* Benzonase® Nuclease is manufactured by Merck KGaA, Darmstadt, Germany and is covered by US Patent 5,173,418 and EP Patent 0,229,866. Nycomed Pharma A/S (Denmark) claims worldwide patent rights to Benzonase® Nuclease, which are licensed exclusively to Merck KGaA, Darmstadt, Germany. Benzonase® Nuclease is a registered trademark of Merck KGaA, Darmstadt, Germany.
Warning: The virus purified using this kit is capable of infecting human or animal cells and could, depending on the gene insert, expose the user to potentially hazardous biological material. Adenoviruses have been designated as Level 2 biological agents. All protocols detailed in these operating instructions must be performed under at least Biosafety Level 2 working condition this kit is NOT intended for human or animal diagnostic or therapeutic applications.