BIOSTAT® B-DCU II


BIOSTAT® B-DCU II
 



 
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The BIOSTAT® B-DCU II is designed for meeting demanding requirements in both research and process development. Unrivaled for scale-down and scale-up modeling of various culture processes, the BIOSTAT® B-DCU II provides highest level of power and flexibility and can be configured with up to six supply towers, each providing independent control of the respective culture vessel.
  • independent parallel process control for up to six supply towers
  • flexible gassing system with up to 6 individual gas outlets including one rotameter per outlet
  • every supply tower allows an independent control of the respective culture vessels, which are in different working volumes, such as 0,5L, 1L, 2L, 5L and 10L, available

System Overview / Supply Tower
The supply tower incorporates all necessary supply devices for independent control of six culture vessels per tower. All culture vessel|supply tower connections are designed for easy handling. For example, quick couplings for vessel jacket and exhaust cooler connections, direct probes and agitation motor connections. Temperature Control: Each culture vessel can be operated and automatically controlled at different temperatures. There are two choices offered. For single wall vessels, there is an electric heating blanket and optional cooling finger with solenoid valve. Alternatively, an integrated, high-efficiency thermostat system – with recirculation pump combined with jacketed culture vessels – features precise temperature control, even at minimal working volumes.
 

System Overview / Dosing Pumps

Up to six industrial proven, easy-to-use peristaltic pumps for each culture vessel are infinitely controlled for addition of corrective agents, feeding, as well as culturing volume control. Up to four of the six aggregates can be analogue speed controlled pumps. Additionally, external pumps for feeding can be easily connected.
 

System Overview / Agitation
The high performance Servo-drive motor assembly combines low shear, gentle agitation for cell cultures and high speed mixing for microbial high cell density cultivation, ensuring high oxygen transfer rates. The motor is gear-free for quiet operation - even at high speed ranges - and provides convenient handling due to the small size.
 

System Overview / Control Performance
The measurement and control capabilities supplied by Sartorius are second to none. The DCU-4 control system belongs to the most proven and advanced bioprocess controllers ever developed. Utilizing proven technology and expert engineering, our existing in-house systems bring powerful control capabilities to the sophisticated biotechnology market. Proven industrial control hardware ensures reliable system performance. The state of the art LAN Supply Tower | Control Tower connection reduces the needed wiring to a minimum. Using a local controller for local process control in combination with a high level SCADA system ensures process control safety – even when PC problems exist.
 

System Overview / Intuitive Touchscreen

The control system presents an intuitive-to-use large TFT colour touch screen for excellent local operation and process control for each of the up to six culture vessels.
Clearly designed screens provide an excellent process value overview and operation. Automatic single or group probe calibration routines reduce the manual operation needed to a minimum.
 

System Overview / UniVessel®
Culture vessels UniVessel® have been developed with over 40 years of experience in sterile and upscale design. Fully-equipped and interchangeable they have been designed to fit all of our autoclaveable Fermentors and Bioreactors.
 
 
  • Available in 0.5L , 1L, 2L, 5L and 10L working volume
  • Single wall or double wall (jacketed) culture vessel with round bottom, baffles and lifting handles
  • Top plate ports for: Media addition | inoculation, 4-way addition, harvest pipe, manual sampler, exhaust cooler, optional cooling finger, probe implementation and aeration fittings
  • Sartorius Stedim gas inlet and exhaust filters
  • pH, pO2, temperature, foam and level probe included
  • Removable holder for up to three storage bottles
  • Stirrer shaft with mechanical seal or magnetic drive
  • 6-blade disc impellers as well as 3-blade segment impellers
  • Aeration tube with ring- or micro-sparger
  • Installation Tubing, o-rings and tool kit

Gassing Strategies / O2 Enrichment

The O2-Enrichment gassing strategy uses a 3-way solenoid valve to select either air or O2 flow to the sparger. Air is the default gas. O2 is pulsed via the solenoid valve, flowing only when required to maintain the dissolved oxygen (DO) setpoint. Air is not provided at this time. A flow meter visually indicates and sets the sparger flow rate. The O2 can be switched from auto control to manual addition. A mass flow controller can be integrated to measure and control the total gas flow range via manual adjustment or automatically in conjunction with the DO controller.
 
 
  • Gas mixing of Air and O2 via solenoid valve
  • Integrated rotameter with manual control valve for gas flow control
  • Optional mass flow controller for total flow
  • Foaming may be reduced due to O2 addition capability
  • Also applicable to sheer stress sensitive cells and filamentous organisms

Gassing strategies/gas flow ratio Control (GFRC)
The Gas Flow Ratio Control gassing strategy utilizes two flow paths, each with mass flow controllers, one for Air and one for O2 flow. Flow meters visually indicate the flow of Air and O2. Both mass flow controllers can be operated manually or automatically in conjunction with the DO controller. The DO controller can be user adjusted to keep e.g. the total flow or the ratio of the Air and O2 constant.
 
 
  • Two integrated mass flow controllers for air and oxygen controlled via DO control loop
  • Integrated rotameter with manual control valve for gas flow control
  • Also applicable to sheer stress sensitive cells and filamentous organisms

Gassing strategies/Advanced Additive Flow - two gas outlets

Advanced Additive Flow - 2 gas outlets gassing strategy allows up to six gas flow paths. Solenoid valves select air, O2, N2 and CO2 for simultaneous flow to the sparger and air to Overlay. Up to six flow meters visually indicate and set the flow rate for each gas. One additional gas flow path can be added to sparger or overlay outlet. Furthermore, two 3-way solenoid valves can be installed to switch the dedicated gas from sparger to overlay (incl. soft switch). The design does support the installation of up to six mass flow controllers, which makes constant sparger gas flow control as well as constant overlay gas flow control possible.
 
 
  • Gas mixing of Air, O2, N2 and CO2 via solenoid valves
  • Up to six integrated Rotameters with manual control valve for sparger or overlay aeration
  • Up to six mass flow controllers for superior control of sparger as well as overlay gasses
  • Easy upgrade of cell culture packages for multi-purpose use
  • For experienced users who need to walk the extra mile in terms of control performance

Gassing Strategies/Advanced Additive Flow - six gas outlets

Advanced Additive Flow - Six gas outlets gassing strategy allows up to six individual gas flow paths. Solenoid valves select air, O2, N2 and CO2 for simultaneous and individual flow. The gas composition to sparger and overlay can be performed outside the gassing system by using T-connectors. Up to six flow meters visually indicate and set the flow rate for each gas. One additional gas flow path can be added. The design does support the installation of up to six mass flow controllers with shut off valves.
 
 
  • Gas mixing of Air, O2, N2 and CO2 via solenoid valves
  • Up to six integrated Rotameters with manual control valve for sparger or overlay aeration
  • Up to six mass flow controllers for superior control of sparger as well as overlay gasses
  • Easy upgrade of cell culture packages for multi-purpose use
  • For experienced users who need to walk the extra mile in terms of control performance

Gassing Strategies / Exclusive Flow
The exclusive flow gassing strategy uses a series of 3-way solenoid valves to select either air, O2, N2 or CO2 to flow the sparger with air as the default. Either Air or CO2 also flows to Overlay. O2, N2 and CO2 can be switched from automatic to manual control. Flow meters for sparger and overlay visually indicate the flow of the selected gas. The design does support the use of mass flow controllers for total flow to sparger and overlay.
 
 
  • Gas mixing of Air, O2, N2 and CO2 via solenoid valves
  • Two integrated Rotameters with manual control valve for total flow sparger and overlay aeration
  • Optional mass flow controllers for total overlay as well as total flow sparger
  • A constant gas flow with quick response controller provides ease of use with flexible adjustments at the same time
  • For beginners as well as experts who want to combine ease of use and flexibility

Supervisory Process Control Software
BioPAT® MFCS provides various solutions to help you at every stage of your process. BioPAT® MFCS | DA is ideal for capturing, storing and visualizing process data from one to four bioprocess units in a research and development setting. BioPAT® MFCS | win is a feature-rich, GAMP category 4 software package capable of supporting the most demanding research or production environment, including S88- and 21CFRpart11-compliance. Both systems are built upon batch-oriented data management, meaning all your process data will be traceable to a unique batch name | ID.